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KMID : 1007520150240020643
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Food Science and Biotechnology
2015 Volume.24 No. 2 p.643 ~ p.650
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Protective Effects of New Blackberry Cultivar MNU-32 Extracts against H2O2-induced Oxidative Stress in HepG2 Cells
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Cho Byoung-Ok
Ryu Hyung-Won
Lee Chang-Wook
Jin Chang-Hyun
Seo Woo-Duck
Ryu Jai-Hyunk
Kim Dong-Sub
Kang Si-Yong
Yook Hong-Sun
Jeong Il-Yun
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Abstract
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Protective effects of extracts of the new cultivar MNU-32 developed from blackberry (Rubus fruticosus) cultivar V-9 against H2O2-induced oxidative stress in HepG2 cells were investigated. DPPH radical scavenging activities of MNU-32 and V-9 extracts were 570.00 μg/mL (IC50) and 644.55 μg/mL (IC50), respectively. Inhibition effects of MNU-32 extracts against intracellular ROS generation, cell cytotoxicity, and lactate dehydrogenase release were higher than for the V-9 extract in H2O2-treated HepG2 cells. Compared with the V-9 extract, MNU-32 extract treatments produced higher levels of lipid peroxidation attenuation and greater protection against DNA damage, restored the activity of superoxide dismutase in H2O2- treated HepG2 cells, and increased protein expression levels of superoxide dismutase in HepG2 cells in a dose and time-dependent manner. MNU-32 blackberry extracts exerted protective effects against H2O2-induced cell damage due to higher anthocyanin contents than the V-9 blackberry extract.
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KEYWORD
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blackberry, anthocyanin, oxidative stress, cell protection, superoxide dismutase
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